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Quantitative Real-Time PCR Detection System

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Labozon Scientific Inc
Quantitative Real-Time PCR Detection System

A Quantitative Real-Time PCR (Polymerase Chain Reaction) Detection System, often abbreviated as qPCR or RT-qPCR, is a sophisticated molecular biology technique used to measure and quantify the amount of DNA or RNA molecules present in a biological sample. This method is widely utilized in various research, clinical, and diagnostic applications due to its sensitivity, specificity, and ability to provide real-time results.


The main components of a typical qPCR detection system include:


Thermal Cycler: The thermal cycler is an essential part of the system, responsible for carrying out the PCR amplification process. It allows precise temperature control to facilitate the different steps of the PCR reaction, namely denaturation, annealing, and extension.


Fluorescent Detection System: The qPCR system is equipped with a fluorescent detection module, usually consisting of an excitation light source and a detector. During the PCR reaction, fluorescent dyes or probes are used to label the amplification products. As the PCR progresses, the detector monitors the increasing fluorescence intensity, and this information is used to measure the amount of DNA or RNA present in the sample.


PCR Plate or Tubes: Samples, along with the necessary reagents (e.g., primers, nucleotides, DNA polymerase), are placed in a PCR plate with multiple wells or individual tubes. This allows for simultaneous analysis of several samples or targets.


Real-Time PCR Detection System ; Sample Volume: 96x0-2-ml-pcr-plate; 12x8-strip-tubes; 96x0-2-ml-single-tube; Range: 1–1010 copies; For more, visit online at Labozon.com!


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