Liposome Preparation: Genizer Liposome Production Solutions

Efficient Liposome Preparation: Genizer Liposome Production Solutions

Liposomes are artificially synthesized vesicles composed of phospholipids and cholesterol that mimic the cellular membrane system, with typical particle sizes ranging from 50–200 nm, adjustable according to preparation methods. They can encapsulate both hydrophilic and hydrophobic active ingredients for targeted delivery, making them ideal carriers for anticancer drugs, gene therapy, and vaccine formulations. Liposomes exhibit excellent biocompatibility and can be functionally modified to extend their capabilities in drug delivery.

Phospholipid bilayer liposomes for gene delivery

In recent years, liposomes have become one of the fastest-growing dosage forms. Known in the industry as “high-end complex injectables,” their unique advantages also require dedicated production equipment and process customization. A critical factor in liposome production is particle size control, which ensures batch-to-batch consistency and optimal drug delivery performance.

Currently, the main methods for reducing liposome particle size include ultrasonication, shearing, high-pressure homogenization (HPH), and extrusion. Genizer primarily focuses on shearing, extrusion, and HPH technologies, covering laboratory research through industrial-scale production.

Liposome Particle Size Reduction Technologies

1. Shearing

Shearing forces are generated as samples pass through narrow gaps between rotors at high speed, refining the particle size. Excessive energy may damage the lipid membrane, so shearing is often used as a pretreatment or for customized liposomes, such as passive drug-loaded liposomes or multilamellar vesicles.

2. High-Pressure Homogenization (HPH)

High-Pressure Homogenization (HPH) is a widely used and reliable method for controlling liposome particle size. As samples pass through the high-pressure interaction chamber, they undergo shear, impact, and turbulence, which rapidly reduce particle size, improve encapsulation efficiency, and ensure batch consistency and stability across both lab-scale and industrial-scale production.

3. Extrusion

Liposome Extrusion Mechanism

Schematic representation of the liposome extrusion process, illustrating how liposomes pass through membranes to achieve controlled particle size.

Extrusion pushes liposomes through a membrane above the phospholipid phase transition temperature, using the pore size and shear forces to control particle size. Optimizing temperature, pressure, and membrane selection can achieve narrow PDI ranges of 0.01–0.03. Extrusion can use single-layer gradient membranes or multi-layer stacked membranes.

Liposome Extrusion Size Distribution

liposome extrusion size distribution particle size uniformity lipid nanoparticle

Liposome size distribution after repeated extrusion passages, demonstrating uniform particle size and narrow PDI for high-quality lipid nanoparticles.

Genizer Liposome Production Equipment

1. Hand Driven Liposome Extruder: Ideal for small-scale laboratory operations, processing 0.1mL–2.5 mL

2. Pneumatic Liposome Extrusion System: Medium-scale continuous production, processing 10mL -10 L

3. High-Pressure Sanitary Pump with Online Liposome Extrusion System: Scalable from lab to industrial scale, producing liposomes with controllable particle sizes of 80–250 nm. Handles volumes from 10 mL to 20 L, and up to 8 extrusion units can be linked to achieve a total processing capacity of 160 L.

4. High-Pressure Homogenizer (HPH): Suitable for rapid homogenization at both lab scale (NanoGenizer Homogenizer) and large-scale production (WILL Series Homogenizer).

Additional Advantages:

Easy to scale: From lab research to full production systems, supporting continuous processing with repeatable results

Temperature control options: Multiple settings including pre-heating and rapid post-process cooling ensure precise particle size control and high encapsulation efficiency

These systems can be combined according to production requirements, providing efficient, controllable, and scalable liposome preparation.

Applications

Liposomes and lipid nanoparticles are used in:

Targeted drug delivery, improving circulation time and stability

Vaccine delivery systems and adjuvants

Anticancer, anti-infection, and immunotherapy drug development

Through particle size, PDI, and circulation adjustments, highly uniform and stable liposomes can be obtained with encapsulation efficiency and active ingredient stability greatly improved.

Explore Genizer Liposome Equipment

Select the ideal system for your lab or production needs:

Hand Liposome Extruder

Pneumatic Liposome Extruder

High Pressure Sanitary Pump + Online Liposome Extruder

High-Pressure Homogenizer (HPH)